Table of Contents
- 1 What is the purple top tube used for?
- 2 What additive is in a yellow-top tube?
- 3 What color tubes are typically spun down?
- 4 Which blood tube is drawn first?
- 5 What is the difference between EDTA and K2 EDTA?
- 6 What color tube is for BMP?
- 7 What does the Purple stopper on a phlebotomy tube do?
- 8 What is in the yellow top of the blood culture tube?
What is the purple top tube used for?
The purple top tube provides blood for infectious disease tests and ascertaining important data such as ABO/Rh (blood type), as well as whether the blood is positive or negative for cytomegalovirus (CMV), HIV, hepatitis, and West Nile virus, to name a few.
What is a yellow-top blood tube for?
Yellow-top tube (ACD) This tube contains ACD, which is used for the collection of whole blood for special tests. NOTE: After the tube has been filled with blood, immediately invert the tube 8-10 times to mix and ensure adequate anticoagulation of the specimen.
What additive is in a yellow-top tube?
acid citrate dextrose
Yellow-top tube: Contains acid citrate dextrose (ACD) solution. Use: ACD whole blood. Send whole blood in a yellow-top tube.
What is a EDTA tube?
EDTA stands for Ethylenediaminetetraacetic acid. EDTA functions by binding calcium in the blood and keeping the blood from clotting. BD Vacutainer Plus Blood Collection Tubes contain K2EDTA, which is spray-dried to the walls of the tube.
What color tubes are typically spun down?
The main difference between the two colors is that the pink top tubes are generally bigger, and get spun down in a centrifuge to separate the plasma from the cells. The pink tubes are primarily used in the blood bank, because we run tests on both the cell part and the plasma part of the blood.
What color tube is drawn last?
1. Collect citrate tube (blue top) last, after 20mL of blood has been withdrawn for other testing, or as waste. Just prior to drawing the blue top tube as the last tube, draw 1mL into a discard blue top tube to prevent cross contamination from the additive of previously drawn tubes.
Which blood tube is drawn first?
EDTA tubes
The order of draw is based on CLSI Procedures and Devices for the Collection of Capillary Blood Specimens; Approved Standard – Sixth Edition, September 2008. This standard recommends that EDTA tubes be drawn first to ensure good quality specimen, followed by other additive tubes and finally, serum specimen tubes.
What is the yellow stuff in the bottom of a blood vial?
The additives may include anticoagulants (EDTA, sodium citrate, heparin) or a gel with density between those of blood cells and blood plasma….Types of tubes.
| Tube cap color or type | Additive | Usage and comments |
|---|---|---|
| Yellow | Acid-citrate-dextrose A (anticoagulant) | Tissue typing, DNA studies, HIV cultures |
What is the difference between EDTA and K2 EDTA?
The main difference between K2 EDTA and K3 EDTA is that K2 EDTA contains two chelated potassium ions whereas K3 EDTA contains three chelated potassium ions.
Why is EDTA tube used for CBC?
Historically, EDTA has been recommended as the anticoagulant of choice for hematological testing because it allows the best preservation of cellular components and morphology of blood cells.
What color tube is for BMP?
Green Tube/Plasma: Centrifuge after collection. Gold Tube/Serum: Allow blood to clot for 30 minutes in a vertical position and centrifuge within 2 hours.
What kind of blood test are purple top tubes used for?
Purple Top Tubes EDTA (Ethylenediaminetetraacetic Acid) tubes use a lavender stopper/shield, white (pearl) shield, pink stopper/shield, royal blue shield or tan shield. These tubes can be used to test CBC (Complete Blood Count), Hematocrit, Hemoglobin, and ESR (Erythrocyte Sedimentation Rate).
What does the Purple stopper on a phlebotomy tube do?
Tubes with purple or lavender-colored stoppers contain EDTA (ethylenediaminetetraacetic acid) additive that binds calcium ions, effectively blocking coagulation cascade. Clinical laboratories use these to test for whole blood.
What are the green top tubes used for?
Green Top Tubes These tubes contain heparin as an additive and may come with or without a gel plasma separator. They may have a green and gray stopper, light green shield, as well as the green shield/stopper. These tubes are used to test ammonia and blood chemistry levels.
What is in the yellow top of the blood culture tube?
Yellow top tube contains sodium polyanethol sulfonate and is used for blood culture specimens or acid-citrate-dextrose (ACD solution A or ACD Solution B). The same tubes are used for HLA phenotyping and for conducting parental tests. Blood culture tubes and vials also come in stoppers other than yellow.