Table of Contents
What may occur if cells are incubated for too long before plating?
When the incubation period is elongated for more than 24-48 hours they will overgrow on plates. Then they will form larger colonies.
What is a disadvantage of the streak plate technique?
What is a disadvantage of the streak plate technique? You can’t accurately count how many colonies there are. What is a disadvantage of the pour plate technique? Sometimes bacteria will clump together and look like a colony when they are not. Will the isolated colonies always be in the fourth sector on the streak plate …
How long do colonies on plates last?
if you are asking about bacterial colony plates, then the cells will be viable for around 2 months, but it must be seal properly using paraffin film to avoid contamination. you have to keep glycerol stock of your cells at -80 or plasmid, which will be safer.
Why are the cells incubated at 37 C?
Mammalian cells operate optimally at 37 oC – molecular kinetics of enzymes and their substrate increase as the temperature increases, meaning a greater number of biochemical reactions can occur. Lower temperatures are less efficient. Higher temperatures cause enzyme denaturation and abruptly halt the progress.
Can bacteria grow on a streak plate?
Can some bacteria grow on the streak plate and not be seen if the pour plate technique is used? Yes, the pour plate is O2 limited. Thus, some bacteria will only grow on the streak plate as it provides ample O2.
Why is bacteria incubated at 30 degrees?
The primary reason for incubating bacterial cultures at different temperatures is that specific bacteria are adapted to grow best at different temperatures.
Can bacteria survive at room temperature?
Bacteria called mesophiles, such as the tuberculosis-causing Mycobacterium tuberculosis, survive best at room temperature and are likely to thrive longer than cold-loving psychrophiles or heat-loving thermophiles. In this way, they can withstand temperature and humidity extremes.